Producción Científica Profesorado

Amperometric biosensor for oxalate determination in urine using sequential injection analysis



Rodríguez Ávila, José Antonio

2012

Rodriguez, J.A., Hernandez, P., Salazar, V., Castrillejo, Y., Barrado, E. Amperometric biosensor for oxalate determination in urine using sequential injection analysis, Molecules, 2012, Vol. 17, p. 8859-8871, ISSN 1420-3049


Abstract


An amperometric flow system for glucose determination in blood serum samples after enzymatic reaction with glucose oxidase immobilized on magnetite covered with silica gel modified propylamine is described. The solid was magnetically retained on a mini-column and placed into the flow injection system preceding the amperometric detector using a modified screen printed electrode with [Fe(tris(3,5-dimetyl-1-pyrazolyl)borate)2]+[FeCl4]-. The variables involved in the system such as flow rate, enzyme concentration, injection volume and reaction coil length were evaluated using a Taguchi parameter design. Under optimal conditions, the calibration curve of glucose sample was linear between 0.24 and 6.00 mM, and with a limit of detection of 0.08 mM. The repeatability for a 4.0 mM glucose solution was 1.0%.The method was validated by comparing the obtained results to those provided by the enzymatic spectrophotometric method; no significant differences were observed.



Producto de Investigación




Artículos relacionados

Presence and antimicrobial resistance of Escherichia coli isolated from foodstuffs in Hidalgo State ...

Magnetic solids in analytical chemistry: A review

Design and Construction of Solid State Ag/AgCl Reference Electrodes Through Electrochemical Depositi...

Determination of tetracyclines in milk samples by magnetic solid phase extraction flow injection ana...

Determination of non-steroidal anti-inflammatory drugs in wastewaters by magnetic matrix solid phase...

Sequential Injection Spectrophotometric Determination of Diclofenac in Urine and Pharmaceutical Form...

Determination of glucose by flow injection analysis with amperometric detection at Fe(III)-(tris(3,5...

Development of a Chloride Ion-Selective Solid State Sensor Based on Doped Polypyrrole-Graphite-Epoxy...

Silver and Silver Chloride Electrodeposits, an Alternative in the Construction of Ag/AgCl Solid Elec...

Synthesis and crystal structures of cis-palladium(II) and cis-platinum(II) complexes containing dipy...