Rotational and Translational Mobility of Small Molecules in Sucrose + Polysaccharide Solutions. 2000. E. Contreras Lopez, D. Champion, H. Hervet, G. Blond and M. Le Meste. Journal of Agricultural and Food Chemistry, 48, 1009-1015.
The effect of different polysaccharides on the rotational (Drot) and translational diffusion (Dtrans) coefficients of small molecules in concentrated systems (sucrose solutions) was investigated. Dextran (1 or 10% w/w) with different molecular masses (from 104 to 2 × 106 Da), gum arabic, or pullulan was added to solutions of sucrose (57.5% w/w). Viscosity measurements of the diffusion medium studied (sucrose and sucrose plus polysaccharide) were made using a Rheometric Scientific viscometer in a temperature range from 20 to ?10 C. The rotational mobility of nitroxide radicals (Tempol) dispersed in the concentrated systems was measured by electron spin resonance. The translational diffusion coefficient of fluorescein was determined by the fluorescence recovery after photobleaching method. The studied temperature range for the latter two techniques was from 20 to ?16 C. For these conditions of concentration and temperature, there was no ice formation in the samples. No effect of the molecular mass of dextran on Drot and Dtrans was observed when solutions with the same dry matter content were compared. Only pullulan and gum arabic, at 10%, had a significant effect on Dtransof fluorescein. Temperature and total dry matter content were observed to be the most important factors controlling Drot and Dtrans in these concentrated systems.