Producción Científica Profesorado

Size exclusion chromatography (SEC-HPLC) as an alternative to study thrombin inhibition



Gonzalez Olivares, Luis Guillermo

2018

Size exclusion chromatography (SEC-HPLC) as an alternative to study thrombin inhibition, Pérez-Escalante, E., González-Olivares, L.G., Cruz-Guerrero, A.E., Galán-Vidal, C.A., Páez-Hernández, M.E., Álvarez-Romero, G.A., Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1074-1075 (2018) 34-38, DOI: 10.1016/j.jchromb.2017.12.037


Abstract


In vitro analysis of anticoagulant compounds with a potential use as antithrombotic drugs, has been traditionally performed using techniques like spectrophotometry, turbidimetry, as well as electrochemical and clinical assays. Although, these techniques have some disadvantages such as: the inability to measure the total biological activity of thrombin, interferences and, sometimes, the quantitative determination of the inhibition ratio is not accurate. In the present work, the conversion of fibrinogen to fibrin was monitored by molecular exclusion chromatography (SEC-HPLC) in three different reaction systems. An inhibition percentage of 43.192.02% was obtained using heparin as an anticoagulant, in addition to the determination of the percentage of heparin bonded to thrombin. This methodology has not been previously described and has high potential for the determination of anticoagulant capacity with higher precision, the determination of thrombin's total biological activity and the quantitative determination of the inhibition ratio



Producto de Investigación




Artículos relacionados

INCORPORACIÓN DE ÁCIDOS GRASOS OMEGA 3 EN HUEVO DE GALLINAS PONEDORAS A TRAVÉS DE LA SUPLEMENTACIÓN ...

Behavior of Salmonella Typhimurium, Staphylococcus aureus, Listeria monocytogenes, and Shigella flex...

Chemical and Physicochemical Properties of Maize Starch After Industrial Nixtamalization

The Avrami Index and the Fractal Dimension in Vegetable Oil Crystallization

Evaluación del Efecto Antihiperglucémico del Bagazo de Naranja (Citrus sinensis var. Valencia) en Es...

EFECTO DEL SOLVENTE ACIDIFICADO SOBRE LAS PROPIEDADES FUNCIONALES DEL ALMIDÓN PRESENTE EN EL MAÍZ AZ...

LIBERACIÓN DE PÉPTIDOS BIOACTIVOS POR BACTERIAS LÁCTICAS EN LECHES FERMENTADAS COMERCIALES

CARACTERIZACIÓN DEL ALMIDÓN DE DOS VARIEDADES DE SORGO DEL ESTADO DE TAMAULIPAS

CALIDAD MICROBIOLÓGICA DE PALETAS BASE AGUA Y BASE LÁCTEA ELABORADAS EN EL ESTADO DE HIDALGO, MÉXICO

Composición química de cebadas cultivadas bajo diferentes condiciones de labranza en tres localidade...