Producción Científica Profesorado

Monoenzymatic Lipase Potentiometric Biosensor for the Food Analysis Based on a pH Sensitive Graphite-epoxy Composite as Transducer



Alvarez Romero, Giaan Arturo

2015

Juan Carlos Escamilla-Mejía, José Antonio Rodríguez, Giaan Arturo Álvarez-Romero, and Carlos Andrés Galán-Vidal, Monoenzymatic Lipase Potentiometric Biosensor for the Food Analysis Based on a pH Sensitive Graphite-epoxy Composite as Transducer, J. Mex. Chem. Soc. 2015, 59(1), 19-23


Abstract


In this paper the development of a potentiometric biosensorbased on the use of the enzyme lipase immobilized in a Nafionmembrane on a graphite-epoxy transducer is reported. This device hasbeen used to quantify triglycerides in food samples from the aqueousextracts obtained by emulsion with Arabic gum. The proposedmethodology does not present significant differences with the spectrophotometricdetermination used as contrast. This work constitutesthe first report of a potentiometric biosensor based on the detection ofchanges of pH using a graphite-epoxy composite transducer and thefirst potentiometric triglyceride biosensor which is not based in ISFETtransducers. The methodology designed is a simple and inexpensivealternative that minimizes the use of organic solvents without affectingthe precision and accuracy of the analysis in complex food samplessuch as butter, chips and pastries.



Producto de Investigación




Artículos relacionados

Prevalence and Antimicrobial Resistance Patterns of Salmonella from Different Raw Foods in Mexico

Magnetic solid phase extraction based on phenyl silica adsorbent for the determination of tetracycli...

Design and Construction of Solid State Ag/AgCl Reference Electrodes Through Electrochemical Depositi...

Chemical studies of anthocyanins: A review

Renewable stationary phase liquid magnetochromatography: determining aspartame and its hydrolysis pr...

Development of a Chloride Ion-Selective Solid State Sensor Based on Doped Polypyrrole-Graphite-Epoxy...

Facilitated transport of Hg(II) through novel activated composite membranes

Potentiometric Behavior of Diverse Polypyrrole-sulphate Films Electro Synthesized on Graphite - Epox...

Chemical studies of anthocyanins: A review

Presence and antimicrobial resistance of Escherichia coli isolated from foodstuffs in Hidalgo State ...