Producción Científica Profesorado

Monoenzymatic Lipase Potentiometric Biosensor for the Food Analysis Based on a pH Sensitive Graphite-epoxy Composite as Transducer



Alvarez Romero, Giaan Arturo

2015

Juan Carlos Escamilla-Mejía, José Antonio Rodríguez, Giaan Arturo Álvarez-Romero, and Carlos Andrés Galán-Vidal, Monoenzymatic Lipase Potentiometric Biosensor for the Food Analysis Based on a pH Sensitive Graphite-epoxy Composite as Transducer, J. Mex. Chem. Soc. 2015, 59(1), 19-23


Abstract


In this paper the development of a potentiometric biosensorbased on the use of the enzyme lipase immobilized in a Nafionmembrane on a graphite-epoxy transducer is reported. This device hasbeen used to quantify triglycerides in food samples from the aqueousextracts obtained by emulsion with Arabic gum. The proposedmethodology does not present significant differences with the spectrophotometricdetermination used as contrast. This work constitutesthe first report of a potentiometric biosensor based on the detection ofchanges of pH using a graphite-epoxy composite transducer and thefirst potentiometric triglyceride biosensor which is not based in ISFETtransducers. The methodology designed is a simple and inexpensivealternative that minimizes the use of organic solvents without affectingthe precision and accuracy of the analysis in complex food samplessuch as butter, chips and pastries.



Producto de Investigación




Artículos relacionados

Nucleation and Growth Kinetics of Electrodeposited Sulfate-Doped Polypyrrole: Determination of the D...

High-performance liquid magneto-chromatography

Development of a Chloride Ion-Selective Solid State Sensor Based on Doped Polypyrrole-Graphite-Epoxy...

Potentiometric behavior of graphite-epoxy electrochemical transducers towards anions, cations and pH...

Determination of primary amino acids in wines by high performance liquid magneto-chromatography

Estudio de las Interacciones Ácido Húmico-Metales Pesados y Determinación de sus Constantes de Estab...

Silver and Silver Chloride Electrodeposits, an Alternative in the Construction of Ag/AgCl Solid Elec...

Validation of a tubular bismuth film amperometric detector: Determination of diclofenac sodium by mu...

Prevalence and Antimicrobial Resistance Patterns of Salmonella from Different Raw Foods in Mexico

Potentiometric Behavior of Diverse Polypyrrolesulphate Films Electro Synthesized on Graphite Epoxy ...