Producción Científica Profesorado

Monoenzymatic Lipase Potentiometric Biosensor for the Food Analysis Based on a pH Sensitive Graphite-epoxy Composite as Transducer



Alvarez Romero, Giaan Arturo

2015

Juan Carlos Escamilla-Mejía, José Antonio Rodríguez, Giaan Arturo Álvarez-Romero, and Carlos Andrés Galán-Vidal, Monoenzymatic Lipase Potentiometric Biosensor for the Food Analysis Based on a pH Sensitive Graphite-epoxy Composite as Transducer, J. Mex. Chem. Soc. 2015, 59(1), 19-23


Abstract


In this paper the development of a potentiometric biosensorbased on the use of the enzyme lipase immobilized in a Nafionmembrane on a graphite-epoxy transducer is reported. This device hasbeen used to quantify triglycerides in food samples from the aqueousextracts obtained by emulsion with Arabic gum. The proposedmethodology does not present significant differences with the spectrophotometricdetermination used as contrast. This work constitutesthe first report of a potentiometric biosensor based on the detection ofchanges of pH using a graphite-epoxy composite transducer and thefirst potentiometric triglyceride biosensor which is not based in ISFETtransducers. The methodology designed is a simple and inexpensivealternative that minimizes the use of organic solvents without affectingthe precision and accuracy of the analysis in complex food samplessuch as butter, chips and pastries.



Producto de Investigación




Artículos relacionados

Mercury Ions Removal from Aqueous Solution Using an Activated Composite Membrane

Synthesis and crystal structures of cis-palladium(II) and cis-platinum(II) complexes containing dipy...

Design and Construction of Solid State Ag/AgCl Reference Electrodes Through Electrochemical Depositi...

Determination of tetracyclines in milk samples by magnetic solid phase extraction flow injection ana...

Development of a Chloride Ion-Selective Solid State Sensor Based on Doped Polypyrrole-Graphite-Epoxy...

Potentiometric behavior of graphite-epoxy electrochemical transducers towards anions, cations and pH...

Potentiometric quantification of saccharin by using a selective membrane formed by pyrrole electropo...

Influence of the Cation Nature of the Sulphate Salt on the Electrochemical Synthesis of Sulfate-Dope...

Characterization of Main Anthocyanins Extracted from Pericarp Blue Corn by MALDI-ToF MS

Magnetic solids in analytical chemistry: A review