Producción Científica Profesorado

Purification of Anthocyanins with oDihydroxyArrangement by Sorption inCationic Resins Charged with Fe(III)



Páez Hernández, María Elena

2014

Purification of Anthocyanins with oDihydroxyArrangement by Sorption inCationic Resins Charged with Fe(III)Castaneda-Ovando, A; Galan-Vidal, CA;Contreras-Lopez, E; Paez-Hernandez,ME(2014) Journal ofChemistry2014 1-9


Abstract


In the present work, a new purification method of anthocyanins with o-dihydroxy arrangement is proposed. This method is based on a ligand-exchange mechanism, using a cationic exchange resin loaded with metallic ions in order to increase the affinity of the resin to the anthocyanin(s) with o-dihydroxy arrangement. This method was used to purify the main anthocyanin (cyanidin-3-glucoside; Cy-3-glc) from the anthocyanic methanolic extract of blue corn. The best sorption result was using Fe(III) in its ion form. The purification procedure begins with the formation of a metal-anthocyanin complex (Cy-3-glc-Fe) which was optimal at pH 5, followed by a NaOH 0.1?M elution process in order to eliminate anthocyanins without o-dihydroxy arrangement, sugars, and organic acids. Finally, the pure anthocyanin is obtained by adding HCl 0.1?M which breaks the metal-anthocyanin complex.



Producto de Investigación




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