Producción Científica Profesorado

Monoenzymatic Lipase Potentiometric Biosensor for the Food AnalysisBased on a pH Sensitive Graphite-epoxy Composite as Transducer



Galan Vidal, Carlos Andres

2015

Juan Carlos Escamilla-Mejía, José Antonio Rodríguez, Giaan Arturo Álvarez-Romero, and Carlos Andrés Galán-Vidal, Monoenzymatic Lipase Potentiometric Biosensor for the Food Analysis Based on a pH Sensitive Graphite-epoxy Composite as Transducer, J. Mex. Chem. Soc. 2015, 59(1), 19-23


Abstract


In this paper the development of a potentiometric biosensorbased on the use of the enzyme lipase immobilized in a Nafionmembrane on a graphite-epoxy transducer is reported. This device hasbeen used to quantify triglycerides in food samples from the aqueousextracts obtained by emulsion with Arabic gum. The proposedmethodology does not present significant differences with the spectrophotometricdetermination used as contrast. This work constitutesthe first report of a potentiometric biosensor based on the detection ofchanges of pH using a graphite-epoxy composite transducer and thefirst potentiometric triglyceride biosensor which is not based in ISFETtransducers. The methodology designed is a simple and inexpensivealternative that minimizes the use of organic solvents without affectingthe precision and accuracy of the analysis in complex food samplessuch as butter, chips and pastries.



Producto de Investigación




Artículos relacionados

Underpotential deposition of cobalt onto polycrystalline platinum

Analysis of the experimental pressuretemperature behavior in the isotropicnematic phase transition f...

Bioacumulación y daños genotóxicos en Pez Cebra (Danio rerio) por arsénico en aguas de Zimapán, Hida...

Quantum chemical study of the electrochemical reduction of the [Co(H2O)6]2+ and [Co(NH3)5(H2O)]2+ io...

Nucleation and growth of cobalt onto different substrates: Part II. The upd-opd transition onto a go...

Morphological and magnetic properties of cobalt nanoclusters electrodeposited onto HOPG

Cobalt electrodeposition onto highly oriented pyrolytic graphite (HOPG) electrode from ammonium sulf...

Revisión de variables de diseño y condiciones de operación en la electrocoagulación

Predicting the Phase Diagram of a Liquid Crystal Using the Convex Peg Model and the Semiempirical PM...

Copper Electrodeposition On Glassy Carbon And Highly Oriented Pyrolytic Graphite Substrates From Per...