Producción Científica Profesorado

Amperometric flow system for blood glucose determination using an immobilized enzyme magnetic reactor.



Rodríguez Ávila, José Antonio

2013

Hernandez, P., Rodriguez, J.A., Galan, C.A., Castrillejo, Y., Barrado, E. Amperometric flow system for blood glucose determination using an immobilized enzyme magnetic reactor. Biosensors and Bioelectronics, 2012, Vol. 41, p. 244-248, ISSN 0956-5663


Abstract


An amperometric flow system for glucose determination in blood serum samples after enzymatic reaction with glucose oxidase immobilized on magnetite covered with silica gel modified propylamine is described. The solid was magnetically retained on a mini-column and placed into the flow injection system preceding the amperometric detector using a modified screen printed electrode with [Fe(tris(3,5-dimetyl-1-pyrazolyl)borate)2]+[FeCl4]-. The variables involved in the system such as flow rate, enzyme concentration, injection volume and reaction coil length were evaluated using a Taguchi parameter design. Under optimal conditions, the calibration curve of glucose sample was linear between 0.24 and 6.00 mM, and with a limit of detection of 0.08 mM. The repeatability for a 4.0 mM glucose solution was 1.0%.The method was validated by comparing the obtained results to those provided by the enzymatic spectrophotometric method; no significant differences were observed.



Producto de Investigación




Artículos relacionados

Determination of non-steroidal anti-inflammatory drugs in wastewaters by magnetic matrix solid phase...

Determination of tetracyclines in milk samples by magnetic solid phase extraction flow injection ana...

Construction and Evaluation of a Gold Tubular Electrode for Flow Analysis: Application to Speciation...

Estudio de las Interacciones Ácido Húmico-Metales Pesados y Determinación de sus Constantes de Estab...

Renewable stationary phase liquid magnetochromatography: determining aspartame and its hydrolysis pr...

Simultaneous determination of tetracyclines in poultry muscle by capillary zone electrophoresis

Sequential Injection Spectrophotometric Determination of Diclofenac in Urine and Pharmaceutical Form...

Characterization of Main Anthocyanins Extracted from Pericarp Blue Corn by MALDI-ToF MS

Synthesis and crystal structures of cis-palladium(II) and cis-platinum(II) complexes containing dipy...

High-performance liquid magneto-chromatography