Producción Científica Profesorado

Determination of quinolones in milk samples using a combination of magnetic solid-phase extraction and capillary electrophoresis



Rodríguez Ávila, José Antonio

2012

Ibarra, I.S., Rodríguez, J.A., Páez-Hernandez, M.E., Santos, E.M., Miranda, J.M. Determination of quinolones in milk samples using a combination of magnetic solid-phase extraction and capillary electrophoresis, Electrophoresis, 2012, Vol. 33, p. 2041-2048, ISSN 0173-0835


Abstract


A magnetic solid-phase extraction (MSPE) method combined with capillary electrophoresis for the simultaneous determination of seven quinolones (QNs) (danofloxacin, ciprofloxacin, marbofloxacin, enrofloxacin, difloxacin, oxolinic acid, and flumequine), using (S)-(+)-6-methoxy- -methyl-2-naphthaleneacetic acid as internal standard, in milk samples was developed. The variables involved in the preconcentration magnetic procedure were: the composition of the magnetic support composition, the sample pH, and the weight of magnetic adsorbent used. The variables were optimized using a simplex-lattice design. Different magnetite covered with octyl-phenyl silica adsorbents were synthesized by varying the molar ratio of phenyltrimethylsilane and octyltrimethoxysilane; the solids were evaluated for QN preconcentration. Under optimal conditions, a linear range was obtained from 27 to 1000 mug L-1 with limits of detection ranging from 9 to 12 mug L?1 for the seven QNs. The absolute recoveries of the seven QNs at three different spiked levels (40, 150, and 400 g L-1) ranged from 74% to 98% with a relative standard deviation less than 10% in all cases. The proposed method was applied to analyze 20 whole milk samples of different brands. All samples were positive for the presence of QN residues; in some cases, extract dilution was required. The concentrations found are in the range from 31.1 to 5047.3 mug L-1. Marbofloxacin was the most frequently found. The method proposed offers advantages in terms of simplicity, sensitivity, efficiency, cost, and analysis time making it an alternative for the analysis of QNs in whole milk samples.



Producto de Investigación




Artículos relacionados

Potentiometric Behavior of Diverse Polypyrrole-sulphate Films Electro Synthesized on Graphite - Epox...

Prevalence and Antimicrobial Resistance Patterns of Salmonella from Different Raw Foods in Mexico

Development of a Polypyrrole Selective Membrane For The Potentiometric Quantification of Saccharinat...

Magnetic solids in analytical chemistry: A review

cis-Palladium(II) complexes of derivatives of di-(2-pyridyl)methane: Study of the influence of the b...

High-performance liquid magneto-chromatography

Determination of oxytetracycline in milk samples by polymer inclusion membrane separation coupled to...

Determination of primary amino acids in wines by high performance liquid magneto-chromatography

Nucleation and Growth Kinetics of Electrodeposited Sulfate-Doped Polypyrrole: Determination of the D...

Synthesis and crystal structures of cis-palladium(II) and cis-platinum(II) complexes containing dipy...