Producción Científica Profesorado

Amperometric flow system for blood glucose determination using an immobilized enzyme magnetic reactor



Galan Vidal, Carlos Andres

2013

Hernandez, P; Rodriguez, JA; Galan, CA; Castrillejo, Y; Barrado, E., Amperometric flow system for blood glucose determination using an immobilized enzyme magnetic reactor BIOSENSORS & BIOELECTRONICS, Año 2013 Volume: 41 Pages: 244-248 DOI: 10.1016/j.bios.2012.08.033


Abstract


An amperometric flow system for glucose determination in blood serum samples after enzymatic reaction with glucose oxidase immobilized on magnetite covered with silica gel modified propylamine is described. The solid was magnetically retained on a mini-column and placed into the flow injection system preceding the amperometric detector using a modified screen printed electrode with [Fe(tris(3,5-dimetyl-1-pyrazolyl)borate)(2)](+) [FeCl4](-). The variables involved in the system such as flow rate, enzyme concentration, injection volume and reaction coil length were evaluated using a Taguchi parameter design. Under optimal conditions, the calibration curve of glucose sample was linear between 0.24 and 6.00 mM, and with a limit of detection of 0.08 mM. The repeatability for a 4.0 mM glucose solution was 1.0%. The method was validated by comparing the obtained results to those provided by the enzymatic spectrophotometric method; no significant differences were observed. (C) 2012 Elsevier B.V. All rights reserved.



Producto de Investigación




Artículos relacionados

Influence of the Crystallinity of the Substrate on Kinetic Parameters of Zinc Electrodeposition Proc...

Hydrogen bond studies in substituted N-(2-hydroxyphenyl)--2-[(4-methylbenzenesulfonyl)amino]acetamid...

Do Spiroarsoranes Exhibit Polytopal Equilibrium in Solution?

Magnetic transition phase diagram of cobalt clusters electrodeposited on HOPG: Experimental and micr...

Estudio de las Interacciones Ácido Húmico-Metales Pesados y Determinación de sus Constantes de Estab...

THEORETICAL STUDY ON THE MODIFICATION OF ACTIVE SITES IN GOLD SURFACES MODELED AS FINITE CLUSTERS: I...

Kinetic study of the cobalt electrodeposition onto glassy carbon electrode from ammonium sulfate sol...

A theoretical quantum study on the distribution of electrophilic and nucleophilic active sites on Ag...

Characterization of Main Anthocyanins Extracted from Pericarp Blue Corn by MALDI-ToF MS

Morphological and magnetic properties of cobalt nanoclusters electrodeposited onto HOPG