Identification of Anthocyanins in Red Grape, Plum and Capulin by MALDI-ToF MS.

A simple and fast method was developed to identify anthocyaninsin red grapes, plums and capulins by MALDI-ToF MS. Threedifferent matrices were used: ?-CHCA (?-cyano-4-hydroxycinnamic acid), THA (2?,4?,6?-trihydroxyacetophenone) and DHBA (2,5-dihydroxybenzoic acid). Post-Source Decay (PSD) fragment detection allowed studying the structure of compounds detected. Deprotonatedanthocyanin species were assigned in capulin ([Cyanidin-3-glucosidedeprotonated]K+ and [Cyanidin-3-rutinoside-deprotonated]K+), due tothese compounds also can be ionized in form of potassium adductscombined with a deprotonation.