Producción Científica Profesorado

Antigenotoxic Effect of Chamomilla recutita (L.) Rauschert Essential Oil in Mouse Spermatogonial Cells, and Determination of Its Antioxidant Capacity in Vitro

Morales González, Jose Antonio


Hernández-Ceruelos A, Madrigal-Santillán E, Morales-González JA, Chamorro-Cevallos G, Cassani-Galindo M, Madrigal-Bujaidar E. Antigenotoxic Effect of Chamomilla recutita (L.) Rauschert Essential Oil in Mouse Spermatogonial Cells, and Determination of its Antioxidant Capacity in vitro. Int J Mol Sci. 2010;11:3793-802. ISSN: 1422-0067


Chamomilla recutita (L.) Rauschert (Asteraceae), popularly known aschamomile, is a plant used in traditional medicine for various therapeutic purposes.Chamomile essential oil (CEO) is particularly known to inhibit the genotoxic damageproduced by mutagens in mice somatic cells. The aim of this research was to determine theinhibitory potential of CEO on the genotoxic damage produced by daunorubicin (DAU) inmice germ cells. We evaluated the effect of 5, 50, and 500 mg/kg of essential oil on therate of sister chromatid exchange (SCE) induced in spermatogonia by 10 mg/kg of themutagen. We found no genotoxicity of CEO, but detected an inhibition of SCE after thedamage induced by DAU; from the lowest to the highest dose of CEO we found aninhibition of 47.5%, 61.9%, and 93.5%, respectively. As a possible mechanism of action,the antioxidant capacity of CEO was determined using the 1,1-diphenyl-2-picrylhydrazyl(DPPH) free radical scavenging method and ferric thiocyanate assays. In the first test weobserved a moderate scavenging potential of the oil; nevertheless, the second assay showedOPEN ACCESSInt. J. Mol. Sci. 2010, 113794an antioxidant capacity similar to that observed with vitamin E. In conclusion, we foundthat CEO is an efficient chemoprotective agent against the damage induced by DAU in theprecursor cells of the germinal line of mice, and that its antioxidant capacity may inducethis effect.

Producto de Investigación UAEH

Artículos relacionados

Inflammation, Oxidative Stress, and Obesity

Hepatoprotective effects of glycine and vitamin E, during the early phase of liver regeneration in t...

In vivo extracellular matrix protein expression by human periodontal ligament after stimulation with...

Exposure to Sodium Fluoride Produces Signs of Apoptosis in Rat Leukocytes

Spasmolytic and anti-inflammatory effects of Aloysia triphylla and citral, in vitro and in vivo stud...

Diabetes. Tratamiento nutricional

Detection of Cytotoxic Activity of Lectin on Human Colon Adenocarcinoma (Sw480) and Epithelial Cervi...

Investigation on the Protective Effect of ?-Mannan against the DNA Damage Induced by Aflatoxin B1 in...

Inflammation, Oxidative Stress, and Obesity

The Combination of Naproxen and Citral Reduces Nociception and Gastric Damage in Rats