2009
Madrigal-Santillán E, Morales-González JA, Sánchez-Gutiérrez M, Reyes-Arellano A, Madrigal-Bujaidar E. Investigation on the protective effect of alpha-mannan against the DNA damage induced by aflatoxin B(1) in mouse hepatocytes. Int J Mol Sci. 2009;10(2):395-406. ISSN: 1422-0067
Abstract
Aflatoxin B1 is a contaminant of agricultural and dairy products that can berelated to mutagenic and carcinogenic effects. In this report we explore the capacity of ?-mannan (Man) to reduce the DNA damage induced by AFB1 in mouse hepatocytes. Forthis purpose we applied the comet assay to groups of animals which were firstadministered Man (100, 400 and 700 mg/kg, respectively) and 20 min later 1.0 mg/kg ofAFB1. Liver cells were obtained at 4, 10, and 16 h after the chemical administration andexamined. The results showed no protection of the damage induced by AFB1 with thelow dose of the polysaccharide, but they did reveal antigenotoxic activity exerted by thetwo high doses. In addition, we induced a co-crystallization between both compounds,determined their fusion points and analyzed the molecules by UV spectroscopy. Theobtained data suggested the formation of a supramolecular complex between AFB1and Man.
The Combination of Naproxen and Citral Reduces Nociception and Gastric Damage in Rats
Lead, Cadmium and Cobalt (Pb, Cd, and Co) Leaching of Glass-Clay Containers by pH Effect of Food
Antinociceptive, genotoxic and histopathological study of Heliopsis longipes S.F. Blake in mice