Producción Científica Profesorado

Exposure to Sodium Fluoride Produces Signs of Apoptosis in Rat Leukocytes



Madrigal Santillán, Eduardo Osiris

2010

Gutiérrez Salinas J, Morales González JA, Madrigal Santillán E, Esquivel-Soto J, Esquivel-Chirino C, García Luna M, Suástegui-Domínguez S, Valadez Vega C. Exposure to Sodium Fluoride Produces Signs of Apoptosis in Rat Leukocytes. Int. J Mol Sci. 2010;11:3610-22. ISSN: 1422-0067.


Abstract


Fluoride is naturally present in the earth's crust and can be found in rocks, coal, and clay; thus, it can be found in small quantities in water, air, plants, and animals. Therefore, humans are exposed to fluoride through food, drinking water, and in the air they breathe. Flouride is essential to maintain bone strength and to protect against dental decay, but if it is absorbed too frequently, it can cause tooth decay, osteoporosis, and damage to kidneys, bones, nerves, and muscles. Therefore, the present work was aimed at determining the effect of intake of sodium fluoride (NaF) as an apoptosis inducer in leukocytes of rats treated for eight weeks with 1 or 50 parts per million (ppm) NaF. Expression of p53, bcl-2, and caspade-3 were used as apoptotic and general metabolism indicators of leukocyte-like indicators of the (INT) oxidation system. Male rats were exposed to NaF (1 and 500 ppm) for eight weeks, and then sacrificed weekly to obtain blood samples. Expression of p53, bcl-2, and caspase-3 were determined in leukocytes by Western blot, and general metabolism of leukocytes was analyzed with a commercial kit. We found changes in theOPEN ACCESSInt. J. Mol. Sci. 2010, 113611expression of the proteins described, especially when the animals received 50 ppm of NaF. These results indicate that NaF intoxication can be an apoptosis inducer in rat leukocytes treated with the compound for eight weeks.



Producto de Investigación UAEH




Artículos relacionados

Protective effect of some vitamins against the toxic action of ethanol on liver regeneration induced...

Exposure to Sodium Fluoride Produces Signs of Apoptosis in Rat Leukocytes

In vivo extracellular matrix protein expression by human periodontal ligament after stimulation with...

Detection of Cytotoxic Activity of Lectin on Human Colon Adenocarcinoma (Sw480) and Epithelial Cervi...

Antinociceptive, genotoxic and histopathological study of Heliopsis longipes S.F. Blake in mice

Diabetes. Tratamiento nutricional

Hepatoprotective effects of glycine and vitamin E, during the early phase of liver regeneration in t...

Antigenotoxic Studies of Different Substances to Reduce the DNA damage Induced by Aflatoxin B1 and ...

Detection of Cytotoxic Activity of Lectin on Human Colon Adenocarcinoma (Sw480) and Epithelial Cervi...

Investigation on the Protective Effect of ?-Mannan against the DNA Damage Induced by Aflatoxin B1 in...