2017
Karina Aguilar-Arteaga, Karla I. De Jesús-Fuentes, Elizabeth Contreras-López, Javier Añorve-Morga,Araceli Castañeda-Ovando, Luis Guillermo González-Olivares, Judith Jaimez-Ordaz, Journal of Food Measurement and Characterization, 2017, 11(2), 879?886.DOI: 10.1007/s11694-016-9459-0
Abstract
In this work, a simple method based on anthocyanins degradation by nitrite ion was used to determine cyclamate in table-top sweeteners. The method was carried out in four steps: (1) cyclamate hydrolysis (hydrolysed cyclamate, HC); (2) reaction between HC and nitrous acid; (3) anthocyanins degradation by residual nitrous acid; and (4) Absorbance measurements at 515 nm. The anthocyanins degradation reaction was done at 20 and 60?C with 3, 5, 7, 10, and 15 mgL?1 HNO2, and several reaction times were tested (15, 30, 45, 60, 90, and 120 min). Under the best conditions (60?C, 10 mgL?1HNO2 and 45 min of reaction time) the calibration curve for cyclamate was linear in the range from 3.19 to 20 mgL?1; the relative standard deviation was 0.62% (for n?=?5) and the limit of detection (LOD) was 0.96 mgL?1. The method was applied for determination of cyclamate in table-top sweeteners and the results were compared with the official method (AOAC 957.09). The effect of synergistic sweeteners (aspartame, acesulfame, sucralose, and saccharin) was also analysed. The results show that the proposed method: (1) reduces considerably the analysis time (from 26 to 2 h, approximately); (2) decreases the LOD and the sample amount is lesser; (3) uses less number of reagents which produces few residues; and (4) it is friendly with the environment, because the anthocyanins source is a plant and the residues can be considered as non-toxics. According with the analysis of synergistic sweetener sacesulfame-K and sucralose present interference; meanwhile aspartame and saccharin (the most used with cyclamate) have not interference in the determination.
Sensory profile and Chemicals composition of Opuntia joconostle from Hidalgo, Mexico
CARACTERIZACIÓN DEL ALMIDÓN DE DOS VARIEDADES DE SORGO DEL ESTADO DE TAMAULIPAS
Chemical and Physicochemical Properties of Maize Starch After Industrial Nixtamalization
The Avrami Index and the Fractal Dimension in Vegetable Oil Crystallization
EFECTO DEL EXTRACTO DE LA CEREZA EN LA REDUCCIÓN DE COLESTEROL.