Carlos A. Gómez-Aldapa, Jesús A. Segovia-Cruz, Jorge F. Cerna-Cortes, Esmeralda Rangel-Vargas, Laura P. Salas-Rangel, Eduardo J. Gutiérrez-Alcántara, Javier Castro-Rosas. 2016. Prevalence and behavior of multidrug-resistant shiga toxin-producing Escherichia coli, enteropathogenic E. coli and enterotoxigenic E. coli on coriander. Food Microbiology. 59(6): 97-103. http://www.sciencedirect.com/science/article/pii/S0740002015300101
The prevalence and behavior of multidrug-resistant diarrheagenic Escherichia coli pathotypes on coriander was determined. One hundred coriander samples were collected from markets. Generic E.coli were determined using the most probable number procedure. Diarrheagenic E.coli pathotypes (DEPs) were identified using two multiplex polymerase chain reaction procedures. Susceptibility to sixteen antibiotics was tested for the isolated DEPs strains by standard test. The behavior of multidrug-resistant DEPs isolated from coriander was determined on coriander leaves and chopped coriander at 252C and 32C. Generic E.coli and DEPs were identified, respectively, in 43 and 7% of samples. Nine DEPs strains were isolated from positive coriander samples. The identified DEPs included Shiga toxin-producing E.coli (STEC, 4%) enterotoxigenic E.coli (ETEC, 2%) and enteropathogenic E.coli (EPEC, 1%). All isolated DEPs strains exhibited multi-resistance to antibiotics. On inoculated coriander leaves stored at 252C or 32C, no growth was observed for multidrug-resistant DEPs strains. However, multidrug-resistant DEPs strains grew in chopped coriander: after 24hat 25 2C, DEPs strains had grown to approximately 3 log CFU/g. However, at 32C the bacterial growth was inhibited. To the best of our knowledge, this is the first report of the presence and behavior of multidrug-resistant STEC, ETEC and EPEC on coriander and chopped coriander.