Producción Científica Profesorado

Peptide separation of comercial fermented milk during refrigerated storage



Añorve Morga, Javier

2014

Peptide separation of comercial fermented milk during refrigerated storage (2014). L. G. González-Olivares, J. Añorve-Morga, A. Castañeda-Ovando, E. Contreras-López, J. Jaimez-Ordaz. Food Sciences and Technology. 34 (4): 644-651. http://dx.doi.org/10.1590/1678-457X.6415


Abstract


Milk is an important source of bioactive compounds. Many of these compounds are released during fermentation and refrigerated storage. The aim of this study was to determine the release of peptides by lactic acid bacteria in commercial fermented milk during refrigerated storage. The size and profile of peptides were analyzed by polyacrylamide gel electrophoresis and sizeexclusion HPLC. During electrophoresis, it was observed that the peptides were released from caseins, whereas β-lactoglobulin was the whey protein with the highest degradation. HPLC analysis confirmed the pattern of peptide formation observed in electrophoresis. Two fractions lower than 2 kDa with aromatic amino acids in their structure were separated. These results were consistent with those reported for structures of peptides with antihypertensive activity. Therefore, the presence of aromatic amino acids in the peptide fractions obtained increases the likelihood of finding peptides with such activity in refrigerated commercial fermented milk. In conclusion, during cold storage, peptides with different molecular weights are released and accumulated. This could be due to the action of proteinases and peptidases of the proteolytic system in lactic acid bacteria.



Producto de Investigación




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