Producción Científica Profesorado

Purification, Biochemical Characterization, and Bioactive Properties of a Lectin Purified from the Seeds of White Tepary Bean (Phaseolus Acutifolius Variety Latifolius)



Villagómez Ibarra, José Roberto

2011

Purification, Biochemical Characterization, and Bioactive Properties of a Lectin Purified from the Seeds of White Tepary Bean (Phaseolus Acutifolius Variety Latifolius), Carmen Valadez-Vega*, Ana María Guzmán-Partida, Francisco Javier Soto-Cordova, Gerardo Álvarez-Manilla, José A. Morales-González, Eduardo Madrigal-Santillán, José Roberto Villagómez-Ibarra, Clara Zúñiga-Pérez, José Gutiérrez-Salinas and Marco A. Becerril-Flores, Molecules, (2011), vol. 16, 2561-2582. , ISSN 1420-3049. Editorial MDPI open Access. País Switzerland. Published online 21 March 2011.


Abstract


The present work shows the characterization of Phaseolus acutifolius variety latifolius, on which little research has been published, and provides detailed information on the corresponding lectin. This protein was purified from a semi-domesticated line of white tepary beans from Sonora, Mexico, by precipitation of the aqueous extract with ammonium sulfate, followed by affinity chromatography on an immobilized fetuin matrix. MALDI TOF analysis of Phaseolus acutifolius agglutinin (PAA) showed that this lectin is composed of monomers with molecular weights ranging between 28 and 31 kDa. At high salt concentrations, PAA forms a dimer of 63 kDa, but at low salt concentrations, the subunits form a tetramer. Analysis of PAA on 2D-PAGE showed that there are mainly three types of subunits with isoelectric points of 4.2, 4.4, and 4.5. The partial sequence obtained by LC/MS/MS of tryptic fragments from the PAA subunits showed 90100% identity with subunits from genus Phaseolus lectins in previous reports. The tepary bean lectin showed lower hemagglutination activity than Phaseolus vulgaris hemagglutinin (PHA-E) toward trypsinized human A and O type erythrocytes. The hemagglutination activity was inhibited by N-glycans from glycoproteins. Affinity chromatography with the immobilized PAA showed a high affinity to glycopeptides from thyroglobulin, which also has N-glycans with a high content of N-acetylglucosamine. PAA showed less mitogenic activity toward human lymphocytes than PHA-L and Con A. The cytotoxicity of PAA was determined by employing three clones of the 3T3 cell line, demonstrating variability among the clones as follows: T4 (DI50 51.5 ?g/mL); J20 (DI50 275 ?g/mL), and N5 (DI50 72.5 ?g/mL).



Producto de Investigación




Artículos relacionados

Estudio químico de cinco plantas mexicanas de uso común en la medicina tradicional

Detection of Cytotoxic Activity of Lectin on Human Colon Adenocarcinoma (Sw480) and Epithelial Cervi...

Antioxidant constituents from Geranium bellum Rose

Purification, Biochemical Characterization, and Bioactive Properties of a Lectin Purified from the S...

Effect of the particle size on the activity of MoOxCy catalysts for the isomerization of heptane

Lead, Cadmium and Cobalt (Pb, Cd, and Co) Leaching of Glass-Clay Containers by pH Effect of Food