Producción Científica Profesorado

Size exclusion chromatography (SEC-HPLC) as an alternative to study thrombin inhibition



Galan Vidal, Carlos Andres

2018

Pérez-Escalante, E., González-Olivares, L.G., Cruz-Guerrero, A.E., Galán-Vidal, C.A., Páez-Hernández, M.E., Álvarez-Romero, G.A., Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, (2018), 34-38. DOI: 10.1016/j.jchromb.2017.12.037


Abstract


In vitro analysis of anticoagulant compounds with a potential use as antithrombotic drugs, has been traditionally performed using techniques like spectrophotometry, turbidimetry, as well as electrochemical and clinical assays. Although, these techniques have some disadvantages such as: the inability to measure the total biological activity of thrombin, interferences and, sometimes, the quantitative determination of the inhibition ratio is not accurate.In the present work, the conversion of fibrinogen to fibrin was monitored by molecular exclusion chromatography (SEC-HPLC) in three different reaction systems. An inhibition percentage of 43.19 2.02% was obtained using heparin as an anticoagulant, in addition to the determination of the percentage of heparin bonded to thrombin.This methodology has not been previously described and has high potential for the determination of anticoagulant capacity with higher precision, the determination of thrombin's total biological activity and the quantitative determination of the inhibition ratio.



Producto de Investigación




Artículos relacionados

Mercury Ions Removal from Aqueous Solution Using an Activated Composite Membrane

Kinetics of Polypyrrole Films Doped with Sulphate Ions Electrodeposited Over Graphite - Epoxy Resin ...

Análisis del Comportamiento Presión-Temperatura en la Transición Isotrópico-Nemático del p-Azoxianis...

Magnetic transition phase diagram of cobalt clusters electrodeposited on HOPG: Experimental and micr...

Effect of TiO2-Al2O3 sol-gel supports on the superficial Ni and Mo species in oxidized and sulfided ...

A theoretical quantum study on the distribution of electrophilic and nucleophilic active sites on Ag...

Zinc Electrodeposition from Chloride Solutions onto Glassy Carbon Electrode

Bioacumulación y daños genotóxicos en Pez Cebra (Danio rerio) por arsénico en aguas de Zimapán, Hida...

Influence of the Cation Nature of the Sulphate Salt on the Electrochemical Synthesis of Sulfate-Dope...

Evaluación de Esteroides Aromáticos como Indicadores de Madurez Térmica en Extractos de Roca