Norberto Chavarría-Hernández, Marco-Antonio Islas-López, Gabriela Maciel-Vergara, Martha Gayosso-Canales, Adriana-Inés Rodríguez-Hernández. 2008. Kinetics of infective juvenile production of the entomopathogenic nematode Steinernema carpocapsae in submerged monoxenic culture. Bioprocess and Biosystems Engineering, 31, 5, 419-426. DOI: 10.1007/s00449-007-0178-0
The effects of culture medium formulations on the kinetics of infective juvenile (IJ) production of the entomopathogenic nematode Steinernema carpocapsae in submerged monoxenic culture, were studied at the cylindrical-bottle scale using six culture media containing agave juice from Agave spp. among other ingredients. The IJ production kinetics was well modelled through a re-parameterised 3-parameter Gompertz model with kinetic parameters: IJ-lag phase ? IJ (day), maximum IJ-stage production rate m max (day?1), and IJ-multiplication factor (C IJ/C IJ,0)max(?). The variation of ? IJ was not very important within fermentations (10.316.2 days); nonetheless, important effects were observed on m max (32.8241.2 days?1) and (C IJ/C IJ,0)max (66(?) to 611.4(?)). Particularly, maximum values of m max and (C IJ/C IJ,0)max were obtained in medium A4 (0.276 l l?1 agave juice, 17 g l?1 yeast extract, 12 g l?1 dried egg yolk, 0.025 l l?1 corn oil). Also, the maximum IJ concentration (249,444 per ml) was achieved in A4-fermentations.