Producción Científica Profesorado

Size exclusion chromatography (SEC-HPLC) as an alternative to study thrombin inhibition



Alvarez Romero, Giaan Arturo

2018

Size exclusion chromatography (SEC-HPLC) as an alternative to study thrombin inhibition, Pérez-Escalante, E., González-Olivares, L.G., Cruz-Guerrero, A.E., Galán-Vidal, C.A., Páez-Hernández, M.E., Álvarez-Romero, G.A., Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1074-1075 (2018) 34-38, DOI: 10.1016/j.jchromb.2017.12.037


Abstract


In vitro analysis of anticoagulant compounds with a potential use as antithrombotic drugs, has been traditionally performed using techniques like spectrophotometry, turbidimetry, as well as electrochemical and clinical assays. Although, these techniques have some disadvantages such as: the inability to measure the total biological activity of thrombin, interferences and, sometimes, the quantitative determination of the inhibition ratio is not accurate. In the present work, the conversion of fibrinogen to fibrin was monitored by molecular exclusion chromatography (SEC-HPLC) in three different reaction systems. An inhibition percentage of 43.192.02% was obtained using heparin as an anticoagulant, in addition to the determination of the percentage of heparin bonded to thrombin. This methodology has not been previously described and has high potential for the determination of anticoagulant capacity with higher precision, the determination of thrombin's total biological activity and the quantitative determination of the inhibition ratio



Producto de Investigación




Artículos relacionados

Development of a novel composite sensor based on doped pyrrole selective to nitrate ions

Validation of a tubular bismuth film amperometric detector: Determination of diclofenac sodium by mu...

Prevalence and Antimicrobial Resistance Patterns of Salmonella from Different Raw Foods in Mexico

Potentiometric behavior of graphite-epoxy electrochemical transducers towards anions, cations and pH...

Magnetic solids in analytical chemistry: A review

Development of a novel nitrate-selective composite sensor based on doped polypyrrole

Facilitated transport of Hg(II) through novel activated composite membranes

Nucleation and Growth Kinetics of Electrodeposited Sulfate-Doped Polypyrrole: Determination of the D...

Potentiometric Behavior of Diverse Polypyrrolesulphate Films Electro Synthesized on Graphite Epoxy ...

Presence and antimicrobial resistance of Escherichia coli isolated from foodstuffs in Hidalgo State ...