Producción Científica Profesorado

Size exclusion chromatography (SEC-HPLC) as an alternative to study thrombin inhibition,



Galan Vidal, Carlos Andres

2018

Size exclusion chromatography (SEC-HPLC) as an alternative to study thrombin inhibition, Pérez-Escalante, E., González-Olivares, L.G., Cruz-Guerrero, A.E., Galán-Vidal, C.A., Páez-Hernández, M.E., Álvarez-Romero, G.A., Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1074-1075 (2018) 34-38, DOI: 10.1016/j.jchromb.2017.12.037


Abstract


In vitro analysis of anticoagulant compounds with a potential use as antithrombotic drugs, has been traditionally performed using techniques like spectrophotometry, turbidimetry, as well as electrochemical and clinical assays. Although, these techniques have some disadvantages such as: the inability to measure the total biological activity of thrombin, interferences and, sometimes, the quantitative determination of the inhibition ratio is not accurate. In the present work, the conversion of fibrinogen to fibrin was monitored by molecular exclusion chromatography (SEC-HPLC) in three different reaction systems. An inhibition percentage of 43.192.02% was obtained using heparin as an anticoagulant, in addition to the determination of the percentage of heparin bonded to thrombin. This methodology has not been previously described and has high potential for the determination of anticoagulant capacity with higher precision, the determination of thrombin's total biological activity and the quantitative determination of the inhibition ratio



Producto de Investigación




Artículos relacionados

Underpotential deposition of cobalt onto polycrystalline platinum

Nucleation and growth of cobalt onto different substrates: Part I. Underpotential deposition onto a ...

Efecto de la composición del soporte sobre las estructuras superficiales de óxidos de níquel y molib...

The role of temperature in copper electrocrystallization in ammoniachloride solutions

Influence of the Cation Nature of the Sulphate Salt on the Electrochemical Synthesis of Sulfate-Dope...

Nucleation and growth of cobalt onto different substrates: Part II. The upd-opd transition onto a go...

Kinetics of Polypyrrole Films Doped with Sulphate Ions Electrodeposited Over Graphite - Epoxy Resin ...

Estudio de las Interacciones Ácido Húmico-Metales Pesados y Determinación de sus Constantes de Estab...

Synthesis of Zn compounds derived from 1H-benzimidazol-2-ylmethanamine

Morphological and magnetic properties of cobalt nanoclusters electrodeposited onto HOPG