2014
Edelmira García-Nieto, Libertad Juárez-Santacruz,Elizabeth García-Gallegos, Joselin Tlalmis-Zempoalteca,Claudia Romo-Gómez, Arturo Torres-Dosal, Bull Environ Contam Toxicol (2014) 93: 393. doi:10.1007/s00128-014-1318-2
Abstract
This study evaluated the genotoxic impact of anthropic activities in Huactzinco Spring, using Cyprinus carpio as a biomonitor. In situ and in vivo experimental designs were compared by means of simultaneous 2-week exposures. The water from the spring generated mean micronuclei frequency values (108.6 32 MN/1,000) and DNA fragmentation values (143.4 35 au) which were statistically higher than those for the negative control (10.9 6 MN/1,000 and 67.6 23 au). The in situ and in vivo experiments supported one another. The comet assay proved to be the most sensitive test, with an EC50 value (11.4 % 3.4 %) being less than that determined for the micronuclei test (54.8 % 3.2 %). The results of this study confirm the usefulness of C. carpio as an environmental contamination biomonitor, and suggest that Huactzinco Spring water constitutes a latent risk to human health and the environment.