Barbosa-Cabrera E, Salas-Casas A, Rojas-Hernández S, Jarillo-Luna A, Abarca-Rojano E, Rodríguez MA, Campos-Rodríguez R. 2012. Purification and cellular localization of the Entamoeba histolytica transcarboxylase. Parasitol Res. 2012 Sep;111(3):1401-5. doi: 10.1007/s00436-012-2898-6. Epub 2012 Mar 28. http://link.springer.com/article/10.1007%2Fs00436-012-2898-6
Genome analysis of Entamoeba histolytica predicts the presence of acetyl-CoA carboxylase. Using Western blot, histochemistry, and confocal microscopy, we demonstrated the presence of a biotin-containing protein in the cytoplasm of E. histolytica, with a molecular weight of 136kDa and biotin-carboxylase activity. This protein probably corresponds to a transcarboxylase that catalyzes the rate-limiting reaction leading to fatty acid elongation.