Producción Científica Profesorado

NADPH oxidase participates in the oxidative damage caused by fluoride in rat spermatozoa. Protective role of a-tocopherol



Sánchez Gutiérrez, Manuel

2011

Izquierdo-Vega JA, Sánchez-Gutiérrez M, Del Razo LM. NADPH oxidase participates in the oxidative damage caused by fluoride in rat spermatozoa. Protective role of a-tocopherol. J Appl Toxicol. 2010 Nov 19. doi: 10.1002/jat.1600. ISSN: 0260-437X.


Abstract


Fluorosis, caused by drinkingwater contaminated with inorganic fluoride, is a public health problem inmany areasaround theworld. The aim of this studywas to evaluate oxidative stress in spermatozoa caused by fluoride and NADPH oxidasein relationship to fluoride. Four experimental groups of maleWistar ratswere administered with deionizedwater, NaF, at a doseequivalent to 5 mg fluoride kg-1 per 24 h, NaF plus 20 mg kg-1 per 24 h a-tocopherol, or a-tocopherol alone for 60 days. Weevaluated several spermatozoa parameters in the four groups: standard quality analysis, superoxide dismutase (SOD) activity,the generation of reactive oxygen species (ROS), NADPH oxidase activity, TBARS formation, ultrastructural analyses ofspermatozoa using transmission electron microscopy and in vitro fertilization (IVF) capacity.After 60 days of treatment, urinaryexcretion of fluoridewas not modified by a-tocopherol. Spermatozoa fromfluoride-treated rats exhibited a significant increasein the generation of ROS, accompanied by a significant increase in NADPH oxidase activity. The increase in ROS generationwassignificantly diminished by diphenylene iodonium, an inhibitor of NADPH oxidase activity. In contrast, a decrease in thegeneration of ROS, an increase in SOD activity and the prevention of TBARS formation process were observed in spermatozoaof rats exposed to fluoride plus a-tocopherol. Finally, a-tocopherol treatment prevented the IVF incapacity observed in thespermatozoa from fluoride-treated rats. These results suggest that NADPH oxidase participates in the oxidative stress damagecaused by subchronic exposure to fluoride. Copyright 2010 JohnWiley & Sons, Ltd.






Artículos relacionados

Effectiveness of Diclofenac, Ketorolac and Etoricoxib in the Treatment of Acute Pain from Ankle Frac...

Pharmacological Interaction between Gabapentin and Glibenclamide in the Formalin Test in the Diabeti...

Investigation on the Protective Effect of ?-Mannan against the DNA Damage Induced by Aflatoxin B1 in...

Decreased in vitro fertility in male rats exposed to fluoride-induced oxidative stress damage and mi...

Antinociceptive, genotoxic and histopathological study of Heliopsis longipes S.F. Blake in mice

Effect of dietary selenium deficiency on the in vitro fertilizing ability of mice spermatozoa

Purification, Biochemical Characterization, and Bioactive Properties of a Lectin Purified from the S...

Pharmacovigilance of Psychoactive Medications in a Mexican Psychiatric Hospital

Presence, processing, and localization of mouse ADAM15 during sperm maturation and the role of its d...

Lead, Cadmium and Cobalt (Pb, Cd, and Co) Leaching of Glass-Clay Containers by pH Effect of Food